Use of Fluorescence Microscopy in an Assay of Sperm Density for the Gorgonian Coral, Plexaura kuna

The density of sperm in the water column during the broadcast spawning events of marine invertebrates is often undetermined or reported in terms of fertilization potential. The density of sperm during such events can be determined by directly counting numbers of spermatozoa using a modification of the acridine orange direct count (AODC) technique for enumerating bacteria. A number of variables in the handling and processing of samples may bias estimates. Sample collection in glass and rapid fixation and filtration are necessary for reproducible estimates. Once filtered, samples are stable for many months, and counts on filters that were poorly stained in the field can be enhanced by additional staining with 4\u276-diamidino-2-phynlindole (DAPI). The AODC technique was employed to examine the effects of distance from a male colony on density of sperm for the gorgonian coral Plexaura kuna. In situ sperm densities ranged from 0 to 1,000 sperm/ml. Sperm densities in 18 l aquaria containing 20 cm long P. kuna explants reached 100,000 sperm/ml

Multicolor Photometry of the Vela Pulsar

Multicolor photometry of the Vela pulsar (PSR B0833-45), updated by recent HST/WFPC2 observations obtained in the 555W, 675W and 814W filters, is presented. The available data provide the best characterization so far of the pulsar spectral shape, which is dominated by a flat power law continuum with spectral index \alpha = -0.2 +/- 0.2, consistent with the extrapolation in the optical domain of the power law component of the X-ray spectrum detected by Chandra. In addition, a marginally significant dip (~ 3 sigma) seems to be present at about 6500 AA. Spectroscopic observations with the VLT, now in progress, will undoubtly provide a much better assessment of the reality and characteristics of this feature.Comment: 4 pages, LaTex, 2 Postscript figures. Astronomy & Astrophysics, accepte

Nothing to hide: An X-ray survey for young stellar objects in the Pipe Nebula

We have previously analyzed sensitive mid-infrared observations to establish that the Pipe Nebula has a very low star-formation efficiency. That study focused on YSOs with excess infrared emission (i.e, protostars and pre-main sequence stars with disks), however, and could have missed a population of more evolved pre-main sequence stars or Class III objects (i.e., young stars with dissipated disks that no longer show excess infrared emission). Evolved pre-main sequence stars are X-ray bright, so we have used ROSAT All-Sky Survey data to search for diskless pre-main sequence stars throughout the Pipe Nebula. We have also analyzed archival XMM-Newton observations of three prominent areas within the Pipe: Barnard 59, containing a known cluster of young stellar objects; Barnard 68, a dense core that has yet to form stars; and the Pipe molecular ring, a high-extinction region in the bowl of the Pipe. We additionally characterize the X-ray properties of YSOs in Barnard 59. The ROSAT and XMM-Newton data provide no indication of a significant population of more evolved pre-main sequence stars within the Pipe, reinforcing our previous measurement of the Pipe's very low star formation efficiency.Comment: Accepted for publication in Ap

The identification of the optical companion to the binary millisecond pulsar J0610-2100 in the Galactic field

We have used deep V and R images acquired at the ESO Very Large Telescope to identify the optical companion to the binary pulsar PSR J0610-2100, one of the black-widow millisecond pulsars recently detected by the Fermi Gamma-ray Telescope in the Galactic plane. We found a faint star (V~26.7) nearly coincident (\delta r ~0".28) with the pulsar nominal position. This star is visible only in half of the available images, while it disappears in the deepest ones (those acquired under the best seeing conditions), thus indicating that it is variable. Although our observations do not sample the entire orbital period (P=0.28 d) of the pulsar, we found that the optical modulation of the variable star nicely correlates with the pulsar orbital period and describes a well defined peak (R~25.6) at \Phi=0.75, suggesting a modulation due to the pulsar heating. We tentatively conclude that the companion to PSR J0610-2100 is a heavily ablated very low mass star (~ 0.02Msun) that completely filled its Roche Lobe.Comment: 17 pages, 5 figures - Accepted for pubblication in Ap

Deep optical observations of the central X-ray source in the Puppis A supernova remnant

X-ray observations reveiled a group of radio-silent isolated neutron stars (INSs) at the centre of young supernova remnants (SNRs), dubbed central compact objects or CCOs, with properties different from those of classical rotation-powered pulsars. In at least three cases, evidence points towards CCOs being low-magnetized INSs, born with slow rotation periods, and possibly accreting from a debris disc of material formed out of the supernova event. Understanding the origin of the diversity of the CCOs can shed light on supernova explosion and neutron star formation models. Optical/infrared (IR) observations are crucial to test different CCO interpretations. The aim of our work is to perform a deep optical investigation of the CCO RX J0822.0-4300 in the Puppis A SNR, one of the most poorly understood in the CCO family. By using as a reference the Chandra X-ray coordinates of RX J0822.0-4300, we performed deep optical observations in the B, V and I bands with the Very Large Telescope (VLT). We found no candidate optical counterpart within 3 sigma of the computed Chandra X-ray position down to 5 sigma limits of B~27.2, V~26.9, and I~25.6, the deepest obtained in the optical band for this source. These limits confirm the non-detection of a companion brighter than an M5 dwarf. At the same time, they do not constrain optical emission from the neutron star surface, while emission from the magnetosphere would require a spectral break in the optical/IR.Comment: 4 pages, 2 figures, Astronomy and Astrophysics, accepte

The Second-Generation Guide Star Catalog: Description and Properties

The GSC-II is an all-sky database of objects derived from the uncompressed DSS that the STScI has created from the Palomar and UK Schmidt survey plates and made available to the community. Like its predecessor (GSC-I), the GSC-II was primarily created to provide guide star information and observation planning support for HST. This version, however, is already employed at some of the ground-based new-technology telescopes such as GEMINI, VLT, and TNG, and will also be used to provide support for the JWST and Gaia space missions as well as LAMOST, one of the major ongoing scientific projects in China. Two catalogs have already been extracted from the GSC-II database and released to the astronomical community. A magnitude-limited (R=18.0) version, GSC2.2, was distributed soon after its production in 2001, while the GSC2.3 release has been available for general access since 2007. The GSC2.3 catalog described in this paper contains astrometry, photometry, and classification for 945,592,683 objects down to the magnitude limit of the plates. Positions are tied to the ICRS; for stellar sources, the all-sky average absolute error per coordinate ranges from 0.2" to 0.28" depending on magnitude. When dealing with extended objects, astrometric errors are 20% worse in the case of galaxies and approximately a factor of 2 worse for blended images. Stellar photometry is determined to 0.13-0.22 mag as a function of magnitude and photographic passbands (B,R,I). Outside of the galactic plane, stellar classification is reliable to at least 90% confidence for magnitudes brighter than R=19.5, and the catalog is complete to R=20.Comment: 52 pages, 33 figures, to be published in AJ August 200

Optical and X-ray observations of candidate isolated neutron stars in the G315.4-2.3 SNR

G315.4-2.3 is a young Galactic supernova remnant (SNR), whose identification as the remains of a Type-II supernova (SN) explosion has been debated for a long time. In particular, recent multi-wavelength observations suggest that it is the result of a Type Ia SN, based on spectroscopy of the SNR shell and the lack of a compact stellar remnant.However, two X-ray sources, one detected by Einstein and ROSAT (Source V) and the other by Chandra (Source N) have been proposed as possible isolated neutron star candidates. In both cases, no clear optical identification was available and, therefore, we performed an optical and X-ray study to determine the nature of these two sources. Based on Chandra astrometry, Source V is associated with a bright V~14 star, which had been suggested based on the less accurate ROSAT position. Similarly, from VLT archival observations, we found that Source N is associated with a relatively bright star ($V=20.14$). These likely identifications suggest that both X-ray sources cannot be isolated neutron stars.Comment: 4 pages, 4 figures, accepted for publication in MNRA

Pictor A (PKS 0518-45) - From Nucleus to Lobes

We present radio and optical imaging and kinematic data for the radio galaxy Pictor A, including HST continuum and [OIII], emission-line images (at a resolution of 25 - 100 mas) and ground-based imaging and spectroscopy (at a resolution of ~ 1.5". The radio data include 3 cm Australia Telescope images of the core, at a resolution comparable to that of the optical, ground-based images, and a VLBI image of a jet in the compact core (at a resolution of 2 - 25 mas), which seems to align with a continuum ``jet'' found in the HST images. The core radio jet, the HST optical continuum ``jet'', and the NW H-alpha filaments all appear to point toward the optical-synchrotron hot-spot in the NW lobe of this object and are associated with a disrupted velocity field in the extended ionized gas. The ground-based spectra which cover this trajectory also yield line ratios for the ionized gas which have anomalously low [NII] (6564), suggesting either a complex, clumpy structure in the gas with a higher cloud-covering factor at larger radii and with denser clouds than is found in the nuclear regions of most NLRG and Seyfert 2 galaxies, or some other, unmodeled, mechanism for the emergent spectrum from this region. The H-alpha emission-line filaments to the N appear to be associated with a 3 cm radio continuum knot which lies in a gap in the filaments ~ 4" from the nucleus. Altogether, the data in this paper provide good circumstantial evidence for non-disruptive redirection of a radio jet by interstellar gas clouds in the host galaxy.Comment: 19 pages, 6 ps.gz fig pages, to appear in the Ap.J. Supp